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为了探究秋水仙素处理对杉木萌动种子的诱变效应,以3种杉木种子为材料,用0.9%的秋水仙素溶液浸泡处理后播种,60d后统计变异苗得率并用流式细胞仪检测变异苗不同部位的细胞倍性。结果表明,经秋水仙素溶液浸泡处理后,3种来源的杉木种子变异苗得率差异显著,全同胞种子Z3#得率最高,为23.45%;3种来源的杉木种子经秋水仙素处理后所得的变异苗均表现为胚根短缩,下胚轴下部明显膨大。流式细胞仪检测表明,胚根部分57.2%的细胞为混倍体细胞,下胚轴下部42.04%的细胞为四倍体细胞,下胚轴中部四倍体细胞所占比例为9.15%,下胚轴上部和子叶部分的细胞均为二倍体细胞;秋水仙素溶液浸泡种子可诱导获得表型特征及细胞倍性发生明显变异的变异苗。本研究结果为进一步开展杉木同源多倍体新种质的创制奠定了基础。
In order to explore the mutagenic effects of colchicine treatment on Chinese fir seeds, three kinds of Chinese fir seeds were used as materials, treated with 0.9% colchicine solution for sowing. After 60 days, the rate of mutant seedlings was counted and the variation of flow cytometry Cells in different parts of the cell ploidy. The results showed that after the soaking with colchicine solution, the seed yield of three sources of Chinese fir was significantly different, and the yield of all-sibling seed Z3 # was the highest (23.45%). After three kinds of Chinese fir seeds were treated with colchicine The resulting mutant showed radicle shortening, lower hypocotyl obvious enlargement. Flow cytometry showed that 57.2% of the cells in the radicle were hybrid somatic cells, 42.04% cells in the lower hypocotyls were tetraploid cells, and the proportion of tetraploid cells in the hypocotyls was 9.15% The cells in the upper part of the hypocotyl and in the cotyledon are all diploid cells. Soaking the seeds with colchicine solution can induce the mutant with obvious phenotype and cell ploidy. The results of this study laid the foundation for the further development of the new plant germplasm with polyploidy of Chinese fir.