论文部分内容阅读
建立准确快速而且不破坏种子的含油量检测方法,对花生品质育种至关重要。本研究利用非破坏性的核磁共振法与索氏提取法对60份黄淮及长江地区主推品种的含油量进行了测定,结果表明两种方法测定结果差异不显著。利用核磁共振法对4个生态环境下种植的60份花生品种进行了种子含油量鉴定,发现含油量在不同环境中表现出极显著差异,其中石家庄种植材料的含油量最高,周口种植材料的含油量最低。综合分析4个环境的鉴定结果,中花16、中花15和冀花11号等3个品种在4个环境中含油量均高于55%。利用195对多态性SSR引物对60份材料进行遗传多样性分析,基因多样性指数和多态信息含量指数(PIC)的平均值分别为0.393和0.349,60份花生品种之间的遗传距离为0.049~0.731,平均为0.388,遗传距离大于0.5的材料所占比重为23.95%。SSR标记与含油量相关性分析结果显示,有11个SSR引物与含油量极显著相关,其中,7个标记位点包括AGGS1280-288、AGGS1442-201、AHGS0288-182、AHGS1578-266、AHGS1891-276、AHGS2036-200和GM1940-122与高含油量相关,这些相关标记和标记位点为花生含油量QTL分析奠定了基础。
Establishing accurate, rapid and seed-free methods for detecting oil content is crucial to quality breeding of peanuts. In this study, non-destructive nuclear magnetic resonance and Soxhlet extraction were used to determine the oil content of 60 main cultivars in Huanghuai and Yangtze River areas. The results showed that there was no significant difference between the two methods. The identification of oil content of 60 peanut cultivars grown in 4 ecological environments by using nuclear magnetic resonance method showed that oil content showed extremely significant difference in different environments, of which the planting oil content in Shijiazhuang was the highest, the oil content in the planting materials in Zhoukou The lowest volume. According to the results of the four environments, the oil content of three cultivars, Zhonghua 16, Zhonghua 15 and Jihua 11, were all higher than 55% in four environments. The genetic diversity of 60 cultivars was analyzed using 195 pairs of SSR primers. The average genetic diversity index (PIC) and polymorphism information content index (PIC) were 0.393 and 0.349 respectively. The genetic distances among 60 peanut cultivars were 0.049 ~ 0.731, with an average of 0.388. The proportion of materials with genetic distance greater than 0.5 was 23.95%. Correlation analysis of SSR markers and oil content showed that there were 11 SSR primers significantly correlated with oil content, of which 7 markers including AGGS1280-288, AGGS1442-201, AHGS0288-182, AHGS1578-266, AHGS1891-276 , AHGS2036-200 and GM1940-122 were correlated with high oil content. These related markers and marker sites laid the foundation for QTL analysis of peanut oil content.