于1.25～1.40 N 硫酸或1.68～1.92 N 高氯酸介质中沸水浴加热40分钟的条件下,赤霉素将12-磷钼杂多酸还原成磷钼杂多蓝,其最大吸收波长,克分子吸光系数和灵敏度分别为710nm,4.398×10~3/·M~(-1)·cm~(-1)和0.4μg/ml。测定波长采用650nm。有色溶液的色泽能稳定160小时以上。赤霉素浓度在0～1 mg/25ml Gibberellic acid heteropolyacid is reduced to phosphomolybdic heteropoly blue by gibberellin under the condition of 1.25 ~ 1.40 N sulfuric acid or 1.68 ~ 1.92 N perchloric acid medium in boiling water bath for 40 minutes, the maximum absorption wavelength, g The molecular absorption coefficient and sensitivity were 710 nm, 4.398 × 10 3 / · M -1 · cm -1 and 0.4 μg / ml, respectively. Determination of wavelength using 650nm. Colored solution can stabilize the color more than 160 hours. Gibberellin concentration 0 ~ 1 mg / 25ml