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目的:研究金铁锁体外抗氧化活性。方法:采用清除采用清除二苯代苦味酰基(DPPH)自由基、清除[2,2’-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基及铁离子还原/抗氧化能力(FRAP)测定法,对金铁锁体外抗氧化活性进行评价,并阳性对照没食子酸丙酯(PG)、丁基羟基茴香醚(BHA)和二丁基羟基甲苯(BHT)比较。结果:金铁锁3个提取物体外抗氧化活性均弱于阳性对照PG,BHA,BHA。在3个提取物中,金铁锁乙酸乙酯提取物清除ABTS自由基(IC50=40.54mg.L-1)及还原Fe3+的能力[TEAC=(696.9±2.42)μmol.g-1]较强;正丁醇清除ABTS自由基(IC50=83.38 mg.L-1)及还原Fe3+的能力[TEAC=(166.1±1.06)μmol.g-1]次之,石油醚提取物最弱。结论:金铁锁乙酸乙酯部位体外抗氧化活性较强。
Objective: To study the anti-oxidative activity of gold lock in vitro. Methods: To remove [2,2’-Amino- (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] (ABTS) by using DPPH radical scavenging The anti-oxidative activities of gold-iron lock were evaluated in vitro and in vitro. The positive control groups, such as propyl gallate, butylated hydroxyanisole (BHA) and dibutylhydroxytoluene BHT) comparison. Results: The anti-oxidative activities of the three extracts of Jin Tie Suo in vitro were weaker than those of PG, BHA and BHA. Among the three extracts, the ethyl acetate extract of Acacia was stronger than that of ABTS (IC50 = 40.54 mg.L-1) and the ability to reduce Fe3 + [TEAC = (696.9 ± 2.42) μmol.g-1] Butanol scavenging ABTS free radical (IC50 = 83.38 mg.L-1) and reducing Fe3 + [TEAC = (166.1 ± 1.06) μmol.g-1] followed by petroleum ether extract was the weakest. Conclusion: The anti-oxidative activity of Ethyl Acetate in vitro is stronger.