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初级三体是用于基因染色体定位的基础材料以芥蓝自交系‘9501’为试材,采用根尖细胞染色体计数法,从其三倍体与二倍体回交子代中筛选出了34个2n+1植株,利用无性快繁技术建成可持续利用的单株试管无性系,经核型分析从中初步获得了4个初级三体(1号初级三体,6 号初级三体,8 号初级三体和随体—初级三体)。各初级三体在株高、株形、叶形等形态特征上表现出一定差异,在减数分裂终变期均以 8Ⅱ+1Ⅲ的联会方式为主,后期Ⅰ染色体呈 9/10 分离的占绝大多数,额外染色体通过雌配子的传递率为 10.15%~27.63%,通过雄配子的传递率为 4.91%~11.86%。
Primary trisomies were used as the basic material for gene chromosomal location. The kale inbred ’9501’ was used as the test material, and the root tip cell chromosome counting method was used to screen out the triploid and diploid backcross generations 34 2n + 1 plants were established, and the sustainable single-plant test tube clones were established by asexual multiplication technique. Four primary trisomies (primary trisomy 1, primary trisomy 6, 8 No. primary trisomy and accompanying - primary trisomy). The primary trisomy showed some differences in plant height, plant shape, leaf shape and other morphological characteristics. In the final stage of meiosis, the association pattern was mainly 8Ⅱ + 1Ⅲ, and the late chromosome Ⅰ was 9/10 The majority of extra chromosomes transfered through female gametes from 10.15% to 27.63% and from male to female through 4.91% to 11.86%.