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通过化学发泡-冷冻干燥-粒子滤出复合法制备聚乳酸(PLLA)大孔支架,然后在大孔内以海藻酸钠(SA)、碳酸钙、葡萄糖酸内酯(GDL)为原料,通过原位相转变制备海藻酸钙水凝胶/聚乳酸复合材料(CA/PLLA);分别利用SEM、压缩强度测试和细胞培养对CA/PLLA支架的形貌、力学性能及生物相容性进行了研究。结果表明:PLLA具有直径小于2mm、孔道相互连通的孔洞,且在大孔中能够形成均匀的CA。CA/PLLA复合材料的压缩强度(2.74MPa)远大于单一的海藻酸钙水凝胶的压缩强度(0.10MPa)。在CA/PLLA复合支架中,软骨细胞呈簇状圆形生长状态,与其在天然软骨陷窝里生长状态一致。这种软硬结合、天然与合成高分子杂化的CA/PLLA复合材料的力学强度和生物相容性同时得到提高,可进一步作为骨和软骨修复材料研究。
Poly (lactic acid) (PLLA) macroporous scaffolds were prepared by chemical foaming-freeze-drying-particle filtration complexation method. Then sodium alginate (SA), calcium carbonate and gluconolactone (GDL) In situ phase transition was used to prepare calcium alginate hydrogel / polylactide composite (CA / PLLA). The morphology, mechanical properties and biocompatibility of CA / PLLA scaffolds were studied by SEM, compressive strength test and cell culture respectively the study. The results show that: PLLA has a diameter of less than 2mm, holes communicating with each other holes, and can form a uniform hole in the large CA. The compressive strength (2.74 MPa) of the CA / PLLA composite is much greater than the compressive strength (0.10 MPa) of a single calcium alginate hydrogel. In the CA / PLLA composite scaffolds, chondrocytes were clustered in a circular pattern, consistent with their growth status in the native cartilage. This combination of hard and soft, natural and synthetic polymer hybrid CA / PLLA composite material simultaneously improve the mechanical strength and biocompatibility, and can be further used as bone and cartilage repair materials.