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了解流行性出血热(HFRS)患者外周血淋巴细胞感染病毒的动态变化,对阐明本病的发病机制有着重要意义。迄今为止,关于患者T、B细胞中病毒抗原检测的报道很少,这可能与细胞的分离、纯化和高质量细胞片的制备较困难有关。我们应用Ficoll-Hypaque和尼龙棉柱法,经过改进,使分
To understand the dynamic changes of peripheral blood lymphocyte infection virus in patients with epidemic hemorrhagic fever (HFRS) is of great significance to clarify the pathogenesis of this disease. To date, little has been reported on the detection of viral antigens in B cells by patient T, which may be related to the difficulty of cell isolation, purification and preparation of high quality cell sheets. We use Ficoll-Hypaque and nylon cotton column method, improved to make points