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目的 探讨卵巢血管紧张素II(AngII)及其受体 (AT1、AT2 )在人卵巢的表达与分布。 方法 免疫组化法检测33份人卵巢组织标本中AngII及其受体AT1、AT2的表达与分布。 结果 AngII及受体AT1、AT2在卵巢的分布基本一致 ,三者在卵母细胞均有表达 ,大卵泡尤其是排卵前卵泡的颗粒细胞含量丰富 ,卵泡膜细胞几无表达 ;颗粒黄体细胞与膜黄体细胞三者含量均丰富 ,并伴随黄体退化而消失。 结论 AngII及其受体可在人卵巢局部生成 ,并存在于同一种细胞内 ,提示它们可能以自分泌方式参与卵巢功能的调节。
Objective To investigate the expression and distribution of ovarian angiotensin Ⅱ (AngII) and its receptors (AT1, AT2) in human ovary. Methods Immunohistochemistry was used to detect the expression and distribution of AngII and its receptors AT1 and AT2 in 33 human ovarian tissues. Results The distribution of Angll and AT1, AT2 in ovary was basically the same, all of them were expressed in oocytes, and the content of granulosa cells in large follicles, especially in preovulatory follicles was abundant, but few in those of follicular cells. Luteal cells were rich in all three, and accompanied by the deterioration of corpus luteum disappeared. Conclusion AngII and its receptors may be localized in human ovary and exist in the same cell, suggesting that they may be involved in the regulation of ovarian function in an autocrine manner.