由于快速简便DNA测序方法的发展,使最近对基因结构和功能的了解有了许多重大进展。Sanger等的双脱氧末端终止法便是一种既简便快速又准确的方法。但此法需要单链DNA模板及特异性引物。已经有一些报道简化了模板和引物的提取步骤。例如,把待测序的DNA片段克隆进双链复制型嗜菌体M13衍生物中,得到单链DNA模板,再与 With the rapid and easy development of DNA sequencing methods, there have been many major advances in the recent understanding of gene structure and function. Sanger et al dideoxy termination method is a simple and fast and accurate method. However, this method requires single-stranded DNA templates and specific primers. There have been several reports that have simplified the template and primer extraction steps. For example, a DNA fragment to be sequenced is cloned into a double-stranded, replication-competent, phage M13 derivative to give a single-stranded DNA template,