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[目的]探讨调节结肠癌细胞中mi R-320a的表达水平后,其对结肠癌细胞生物学行为的影响及可能作用机制。[方法]检测调节mi R-320a结肠癌细胞的表达水平后,用MTT法检测细胞增殖能力的变化,Transwell侵袭实验检测细胞侵袭力变化,通过给裸鼠尾静脉注射mi R-320a不同表达水平的结肠癌细胞后,观察裸鼠肺转移的情况;通过生物信息学软件预测mi R-320a的潜在靶基因并通过双荧光素酶报告基因系统进行鉴定,Western Blot法检测β-catenin、MMP-2、MMP-9、E-cadherin、N-cadherin和Vimentin的蛋白表达情况。[结果 ]通过慢病毒感染mi R-320a质粒上调结肠癌细胞中mi R-320a水平后,可以抑制结肠癌细胞增殖、抑制结肠癌细胞侵袭及肺转移,β-catenin是mi R-320a的靶基因,mi R-320a下调β-catenin、MMP-2、MMP-9、N-cadherin和Vimentin表达,上调E-cadherin表达。[结论 ]mi R-320a可以抑制结肠癌细胞的增殖及侵袭转移。
[Objective] To investigate the effect of mi R-320a on the biological behavior of colon cancer cells and its possible mechanism after regulating the expression of mi R-320a in colon cancer cells. [Methods] The expression of mi R-320a in colon cancer cells was detected by MTT assay. The cell invasiveness was detected by Transwell invasion assay. The expression of mi R-320a Of colon cancer cells were observed after the transfer of lung metastases in mice; bioinformatics software to predict the potential of mi R-320a target gene and dual luciferase reporter gene system identification, Western Blot detection of β-catenin, MMP- 2, MMP-9, E-cadherin, N-cadherin and Vimentin protein expression. [Results] mi R-320a plasmid could up-regulate the expression of mi R-320a in colon cancer cells by lentivirus infection, which can inhibit the proliferation of colon cancer cells, inhibit the invasion of colon cancer cells and lung metastasis, and β-catenin is the target of mi R-320a Mi R-320a down-regulates the expression of β-catenin, MMP-2, MMP-9, N-cadherin and Vimentin and upregulates the expression of E-cadherin. [Conclusion] mi R-320a can inhibit the proliferation, invasion and metastasis of colon cancer cells.