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目的:了解正常人口腔黏膜全基因组甲基化状态。方法:应用甲基化DNA免疫沉淀技术检测正常人口腔黏膜全基因组甲基化。结果:正常人口腔黏膜中检测出的甲基化CpG岛占总数的16.4%,其中,位于基因启动子区段的甲基化CpG岛占40.2%,甲基化CpG岛和甲基化基因均主要集中于1、2、16、17、19号染色体,甲基化的基因主要集中在与癌症、MAPK、钙信号相关的通路中。结论:人正常口腔黏膜存在甲基化现象,甲基化CpG岛主要集中在富含基因的染色体上。
Objective: To understand the normal methylation status of oral mucosa in normal people. Methods: Methylation of oral mucosa was detected by methylation DNA immunoprecipitation. Results: The methylation CpG islands detected in the oral mucosa of normal people accounted for 16.4% of the total, of which 40.2% were methylated CpG islands located in the gene promoter region, and both methylated CpG islands and methylated genes Mainly on chromosomes 1, 2, 16, 17 and 19. The methylation genes are mainly concentrated in the pathways related to cancer, MAPK and calcium signaling. Conclusion: There is methylation in normal human oral mucosa. Methylated CpG islands are mainly concentrated on chromosomes rich in genes.