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目的建立活血止痛胶囊中土鳖虫的一种分子鉴定方法。方法采用蛋白酶K法结合吸附柱法提取活血止痛胶囊中土鳖虫基因组DNA,使用蜚蠊目昆虫通用引物扩增其线粒体16S rRNA片段并测序。结果土鳖虫能扩增出475 bp左右的片段,本实验成功从自制活血止痛胶囊中扩增出该片段。结论该方法灵敏度高,可靠性强,可以做为活血止痛胶囊中土鳖虫的一种鉴定方法。
Objective To establish a molecular identification method of Eupolyphaga sclerotiorum in Huoxuezhitong capsule. Methods The genomic DNA of Eupolyphaga sinensis in Huoxuezhitong Capsules was extracted by proteinase K method and adsorption column method. The mitochondrial 16S rRNA fragment was amplified by using the common primers of Blattella spp. And sequenced. Results Eupolyphaga sinensis could amplify a fragment of about 475 bp. The experiment successfully amplified this fragment from Huoxue Zhitong capsule. Conclusion The method is highly sensitive and reliable, and can be used as a method to identify Eupolyphaga sclerotiorum in Huoxuezhitong capsule.