中国野生大豆对斜纹夜蛾的抗生性鉴定及资源遴选

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试验在前期对栽培大豆进行抗生性鉴定的基础上,2014和2015年在养虫室喂养斜纹夜蛾初孵幼虫,以喂养第6,9和12天幼虫重的隶属函数值为抗生性指标评价了来自我国各大豆生态区的200份代表性野生大豆种质资源的抗虫性。两年第6天结果的联合方差分析表明:材料间、年份间、材料与年份互作间差异均达极显著水平。2015年不同喂养期幼虫重的隶属函数值联合方差分析表明:材料间、喂养期间差异均达极显著水平,材料与喂养期间无明显互作。按标准品种分级法对供试材料进行分级,发现野生大豆抗生性与地理来源有关,长江中下游生态区(III)及西南-中南生态区(IV)的高抗材料较多,而北方生态区(I)的高感材料较多。从供试的野生大豆资源中筛选出高抗和高感材料各10份,高抗材料的抗性高于国际常用的栽培大豆抗源PI227687和Lamar,可用于后续的野生大豆抗虫鉴定、抗虫机理及抗虫育种等研究。 Based on the early identification of the cultivated soybean, the membership functions of the larvae of Spodoptera litura were fed on the 6th, 9th and 12th day in 2014 and 2015 respectively. The membership values ​​of the larvae were evaluated as antibiotic markers The insect resistance of 200 representative wild soybean germplasm resources from all soybean eco-regions in China was studied. A joint variance analysis of the results at the 6th day of the two years showed that there was a significant level of discrepancy between material, year, material and year. The analysis of membership functions of different feeding larvae in 2015 showed that there was significant difference between the materials during the feeding period and no significant interaction between the materials and the feeding period. According to the standard variety grading method, the tested materials were graded and found that the antibiotic resistance of wild soybean was related to geographical origin. There were more high resistant materials in the middle and lower reaches of the Yangtze River (III) and southwest-central-southern ecological zone (IV) (I) high sense of material more. Ten high and high sensitive materials were screened from the tested wild soybean resources. The resistance of the high resistant material was higher than that of the international cultivated soybean resistance PI227687 and Lamar, which could be used for the subsequent identification of wild soybean resistance. Insect mechanism and insect breeding and other research.
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