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目的对仅发生过一次无保护肛交男男同性恋者进行追踪随访和检测以判定其是否感染HIV,并在随访过程中采集血清保存。方法发生高危肛交性行为后对该患者进行4次追踪检测,抽血进行HIV(human immunodeficiency virus,HIV)抗体筛查和确证试验、CD4~+T淋巴细胞检测以及病毒载量检测,梅毒螺旋体抗原血清检测。结果 HIV抗体酶联免疫吸附试验(ELISA)检测值/临界值(S/CO)持续上升,HIV-1抗体蛋白印迹法(WB)出现的条带越来越多直至全带型,病毒载量检测值越来越高至5600cp/mL,最终诊断为HIV感染,CD4~+T淋巴细胞计数随着HIV-1病毒载量的上升应激性升高,梅毒螺旋体抗原血清检测阴性。结论对高危人群HIV抗体不确定者的追踪随访至关重要,规范采集保存此类血清标本具有极高的研究价值。
Objective To follow-up and test only one unprotected gay male gay man to determine whether it is HIV-infected and to collect serum during follow-up. Methods Four follow-up tests were performed on the patients after high-risk anal intercourse. Blood was collected for HIV antibody screening and confirmatory tests, CD4 ~ + T lymphocyte detection and viral load testing. Treponema pallidum antigen Serum detection. Results The detection value / critical value (S / CO) of HIV antibody ELISA continued to increase, and more and more bands appeared in the Western blotting of HIV-1 antibody (WB) The detection value is getting higher and higher to 5600cp / mL. Finally, the diagnosis of HIV infection, CD4 ~ + T lymphocyte count increased with HIV-1 viral load increased, serum test of Treponema pallidum antigen negative. Conclusion It is very important to follow up and follow up the uncertainties of HIV antibody in high-risk population. It is of great value to standardize the collection and preservation of such serum samples.