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当长期持续注入使用5-氟脲嘧啶(5-FU)时,为了判断其在体内的药物动力学,我们研究出了一种快速和灵敏的HPLC分析方法,用于人血清中5-FU的定量. 5-氟脲嘧啶和氯脲嘧啶购自Sigma化学公司(st.Louis,MO).所有化学试剂均为分析纯. 使用改进两个已报道的HPLC分析系统来完成血清中5-FU的定量。换句话说,用于5-FU的测定有:一个Beckman 110-A型泵(Beckman Instruments,Berkeley,CA),并带有一根Lich-
To determine its pharmacokinetics in vivo when 5-fluorouracil (5-FU) was continuously infused over a long period of time, we developed a rapid and sensitive HPLC analysis method for 5-FU in human serum Quantitative 5-fluorouracil and chlorouracil were purchased from Sigma Chemical Co. (st.Louis, MO) All chemicals were of analytical grade Two of the reported HPLC analytical systems were modified to complete the 5-FU in serum Quantitative. In other words, the assay for 5-FU was a Beckman Model 110-A pump (Beckman Instruments, Berkeley, CA) with a Lich-