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目的 探讨急性呼吸道感染 (ARI)患儿的肠道病毒 (EV)感染情况。方法 取 2 0 0 2年 2～ 11月住院的10 0例ARI患儿的鼻咽深部分泌物 ,用逆转录聚合酶链反应 (RT PCR)法检测EV ,并用间接免疫荧光法及病毒分离检测 7种常见呼吸道病毒。结果 10 0例患儿中EVRT PCR阳性 4 6例 ,占 4 6 % ,其中 3岁以下婴幼儿 34例 ,占73 9% (34/ 46 ) ;2月、5月、6月、7月及 9月EV阳性率较高 ,为 5 0 %～ 10 0 % ,8月和 10月EV阳性率分别为 4 0 0 %和 4 1 7% ,11月EV阳性率相对较低 ,为 17 6 % ;EV阳性患儿中 ,5例合并呼吸道合胞病毒感染 ,1例合并副流感病毒 3型感染 ,这 6例均为冬季 (2月和 11月 )住院的患儿。单纯EV阳性患儿中 85 0 % (34/ 40 )伴有腹泻 ,EV阴性者中 4 0 7% (2 2 / 5 4 )伴有腹泻 ,差异有显著性 ,但 1～ 6个月小婴儿单纯EV阳性者与EV阴性者伴腹泻的比例差异无显著性。结论 EV是儿童ARI不可忽视的病原之一 ,其中婴幼儿EV感染占多数 ;夏秋季EV感染率较高 ,冬季EV常与其他呼吸道病毒混合感染 ;EV感染时较易出现腹泻 ,6个月以上儿童伴有腹泻为EV感染的特点之一。
Objective To investigate the enterovirus (EV) infection in children with acute respiratory infection (ARI). Methods Nasopharyngeal secretions from 100 children with ARI who were hospitalized from February to November in 2002 were retrospectively analyzed. The EV was detected by reverse transcriptase-polymerase chain reaction (RT PCR) and detected by indirect immunofluorescence and virus isolation Seven common respiratory viruses. Results Forty cases of EVRT PCR were found in 40 cases, of which 34 (46%) were infants under 3 years of age, accounting for 73.9% (34/46). In February, May, June, July and The positive rate of EV in September was high from 50% to 100%. The positive rates of EV in August and October were 40% and 41.7% respectively. The positive rate of EV in November was relatively low at 17 6% Five of the EV-positive children had respiratory syncytial virus infection and one had the parainfluenza virus type 3 infection. All 6 were hospitalized in winter (February and November). Diarrhea was found in 85 0% (34/40) of EV-positive children and 40.7% (2 of 52/4) of EV-negative patients with diarrhea, but the difference was significant There was no significant difference in the proportion of diarrhea between EV-positive patients and EV-negative patients. Conclusion EV is one of the most important pathogens in children with ARI. EV infection in infants and young children accounts for the majority of cases; EV infection rate is higher in summer and autumn, EV infection in winter is often mixed with other respiratory viruses; diarrhea is more likely to occur in EV infection. After 6 months Children with diarrhea is one of the characteristics of EV infection.