对比研究了不同培养时期添加生物素和三磷酸腺苷(ATP)对毒三素链霉菌脂类代谢的影响。结果表明,无论在培养起始阶段还是产物合成阶段,随着生物素添加浓度的增加,利普斯他汀的产量降低,胞内油脂含量增加。如在培养起始阶段添加1.2 mg/L生物素时,利普斯他汀产量降低28.9%,总油脂量提高至15.8%。相反,在培养起始阶段和产物合成期添加高浓度ATP,会促进利普斯他汀的合成,抑制胞内总油脂的积累。而且在产物合成阶段添加生物素和ATP对菌体代谢的影响大于在起始阶段添加。 The effects of adding biotin and adenosine triphosphate (ATP) on the lipid metabolism of Streptomyces albicans at different culture stages were compared. The results showed that, with the increase of the concentration of biotin, the production of lipstatin decreased and the content of intracellular lipid increased in both the initial stage of culture and the stage of product synthesis. When 1.2 mg / L biotin was added in the initial stage of culture, the yield of Liupressin decreased by 28.9% and the total fat content increased to 15.8%. In contrast, the addition of high concentrations of ATP in the initial stages of culture and in the product-synthesis period promotes the synthesis of lispastat and inhibits the accumulation of intracellular total lipids. Moreover, adding biotin and ATP during the process of product synthesis has more influence on the metabolism of the cells than adding at the initial stage.