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目的:明确FAP发挥促增殖、侵袭和迁移作用的细胞内传递途径。方法:Transwell实验和迁移实验检测FAP、RhoA/ROCK、Rac1-GTP对卵巢癌细胞系HO-8910PM的增殖,侵袭和迁移的影响。结果:迁移和侵袭实验证实用Y-27632抑制RhoA/ROCK途径能促进卵巢癌细胞的增殖、迁移和侵袭,与FAP联合作用时可使促进作用增强。迁移和侵袭实验证实NSC23766可抑制Rac1迁移和侵袭,与FAP联合作用可使FAP的促进作用减弱。结论:FAP不是通过细胞内RhoA/ROCK,而是通过Rac1-GTP信号通路在HO-8910PM细胞发挥促增殖、迁移和侵袭作用的。
Objective: To clarify the FAP play a role in promoting proliferation, invasion and migration of intracellular transmission. Methods: The effects of FAP, RhoA / ROCK and Rac1-GTP on the proliferation, invasion and migration of ovarian cancer cell line HO-8910PM were detected by Transwell assay and migration assay. Results: Migration and invasion assays demonstrated that the inhibition of RhoA / ROCK pathway by Y-27632 can promote the proliferation, migration and invasion of ovarian cancer cells. Migration and invasion experiments confirmed that NSC23766 can inhibit Rac1 migration and invasion, combined with FAP FAP can promote the role of weakened. CONCLUSION: FAP does not promote the proliferation, migration and invasion of HO-8910PM cells through Rac1-GTP signaling through intracellular RhoA / ROCK.