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目的探讨男性尿道口细胞中人乳头瘤病毒(HPV)不同基因型别的感染情况及其临床意义。方法从304例男性尿道口细胞标本中提取23种HPV DNA,采用基因扩增结合基因芯片杂交技术对其进行HPV基因检测,并对受检者行基因型别的分析。结果 304例男性受检者中检出HPV感染者117例,总阳性率为38.49%(117/304)。其中单一型别的HPV感染者75例,占24.67%(75/304),单一型别的感染中,HPV6型感染者26例,阳性率为34.67%(26/75),是单一型别最主要的感染类型,其次分别为HPV11型10例、16型7例、58和81型各6例、33型5例,感染率分别为13.33%(10/75)、9.33%(7/75)、8.00%(6/75)、8.00%(6/75)、6.67%(5/75)。多型HPV感染者42例,占13.82%(42/304),其中二重感染者34例,占多型感染的80.95%(34/42),三重感染者6例,占多型感染的14.29%(6/42)。HPV感染的年龄组分布比较差异无统计学意义(P>0.05)。结论 HPV 6型、11型、16型、58型、81型、33型是男性尿道口细胞感染的主要基因型别,基因扩增结合基因芯片检测技术是一种比较适合临床对男性进行HPV分型检测的敏感性高、特异性强的诊断方法,尤其适合开展男性尿道口细胞HPV感染的分子流行病学的研究。
Objective To investigate the infection status of human papillomavirus (HPV) genotypes in male urethral orifice cells and its clinical significance. Methods Totally 23 kinds of HPV DNA were extracted from 304 specimens of male urethral orifice cells. HPV gene was detected by gene amplification and gene chip hybridization, and genotypes of the subjects were analyzed. Results Among 304 male subjects, 117 cases were detected with HPV infection, the total positive rate was 38.49% (117/304). Among them, 75 cases were single-type HPV infection, accounting for 24.67% (75/304). In the single-type infection, 26 cases were HPV6 infection, the positive rate was 34.67% (26/75) The main infection types were HPV11, 7, 16, 58, 81 and 5, respectively. The infection rates were 13.33% (10/75) and 9.33% (7/75) , 8.00% (6/75), 8.00% (6/75), 6.67% (5/75). Twenty-four cases of multi-type HPV infection accounted for 13.82% (42/304), of which 34 cases were double infection, accounting for 80.95% (34/42) of multi-type infection, 6 cases were triple infection, accounting for 14.29 cases of multi-type infection % (6/42). There was no significant difference in age distribution among HPV infection (P> 0.05). Conclusion HPV type 6, type 11, type 16, type 58, type 81 and type 33 are the major genotypes of male urethral cell infection. Gene amplification combined with gene chip detection is a more suitable method for clinical diagnosis of HPV in men Type detection of high sensitivity and specificity of the diagnostic method, especially for the development of male urethral cell HPV infection molecular epidemiology.