论文部分内容阅读
目的比较溃疡性结肠炎患者与健康人肠道黏膜组织的分子信号通路基因表达差异,了解溃疡性结肠炎信号调控的分子机制。方法收集北京协和医院2011—2012年收治的3例溃疡性结肠炎患者和3名健康对照组相同部位结肠黏膜组织。组织提取RNA,应用逆转录试剂盒转录为c DNA,应用RT2Profiler TM PCR Array基因表达芯片,包含84个信号传导通路基因。结果与健康对照组相比,溃疡性结肠炎组织9个基因表达上调,9个基因表达下调,66个基因表达无显著改变。其中FOS、EGR1、DUSP1、MMP7表达显著上调。溃疡性结肠炎组织IKB、NF-κB信号通路中,多个基因表达下调,包括CHUK、FASLG、STAT1。结论溃疡性结肠炎发病与EGF、IKB等多个信号传导通路相关。其中FOS和EGR1是溃疡性结肠炎发病机制中的重要转录因子。
Objective To compare the differences in the expression of molecular pathways between ulcerative colitis and healthy human intestinal mucosa and to understand the molecular mechanism of ulcerative colitis signal regulation. Methods The colonic mucosa tissues of 3 patients with ulcerative colitis and 3 healthy controls in Beijing Union Medical College Hospital from 2011 to 2012 were collected. Tissue RNA was extracted and transcribed into cDNA using a reverse transcription kit. The RT2Profiler ™ PCR Array gene expression chip was used and contained 84 signal transduction pathway genes. Results Compared with healthy control group, the expression of 9 genes in ulcerative colitis was up-regulated and the expression of 9 genes was down-regulated. There was no significant change in 66 genes. Among them, the expression of FOS, EGR1, DUSP1 and MMP7 were significantly up-regulated. In ulcerative colitis IKB, NF-κB signaling pathway, multiple genes downregulation, including CHUK, FASLG, STAT1. Conclusions The incidence of ulcerative colitis is related to multiple signal transduction pathways such as EGF and IKB. Among them, FOS and EGR1 are important transcription factors in the pathogenesis of ulcerative colitis.