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目的:探讨肠舒颗粒对腹泻型肠易激综合征(IBS)大鼠血中血管活性肠肽(VIP)、P物质(SP)的影响。方法:将40只大鼠随机分为空白组、模型组、肠舒颗粒组、氯化钠注射液组、模型组。采用“精神刺激+饮食失宜+劳倦”方法建立肝郁脾虚型IBS动物模型。模型建立后,空白组、模型组给予正常喂养,肠舒颗粒组与氯化钠注射液组分别给予相应药物灌胃。采用酶联免疫法测定血中VIP、SP水平。结果:模型组VIP、SP水平高于空白组、肠舒颗粒组(P<0.05或P<0.01);肠舒颗粒组与空白组比较,差异无统计学意义(P>0.05);但与氯化钠注射液组比较,差异有统计学意义(P<0.05)。结论:肠舒颗粒通过减少胃肠激素分泌释放而抑制IBS(腹泻型)的肠运动功能,为临床治疗腹泻型IBS提供了实验依据。
Objective: To investigate the effect of Changshu Granule on vasoactive intestinal peptide (VIP) and substance P (SP) in the blood of diarrhea-predominant irritable bowel syndrome (IBS) rats. Methods: Forty rats were randomly divided into blank group, model group, Changshu granule group, sodium chloride injection group and model group. The model of IBS animal model of liver depression and spleen deficiency was established by using the method of “Mental stimulation + diet loss + fatigue”. After the model was established, the blank group and the model group were given normal feeding, and the corresponding drugs were given to the gut Shu granules group and the sodium chloride injection group respectively. Serum VIP and SP levels were measured by enzyme-linked immunosorbent assay. Results: The levels of VIP and SP in model group were significantly higher than those in blank group and Changshu granule group (P <0.05 or P <0.01). There was no significant difference between Changshu granule group and blank group (P> 0.05) Sodium injection group, the difference was statistically significant (P <0.05). Conclusion: Granule can inhibit the intestinal motility of IBS by decreasing the secretion of gut hormones and provide the experimental basis for the clinical treatment of diarrhea-type IBS.