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为明确海南菠萝心腐病菌烟草疫霉的生物学特性,研究不同培养基、温度、pH值和光照对病菌菌体生长和孢子囊产生的影响。结果表明,V8汁、胡萝卜两种培养基适宜菌丝生长,而V8汁、玉米粉和番茄3种培养基适合病菌产生孢子囊,尤其是番茄琼脂培养基,可诱导病菌产生大量孢子囊。病菌在10~35℃范围内均可生长,最适温度范围是24~32℃。最适pH值为5~8,在pH值7~8稍偏碱时,菌丝生长速度最快。光照条件对菌丝生长影响不明显,但明显促进孢子囊的产生。
In order to clarify the biological characteristics of Phytophthora nicotianae in Hainan pineapple fungus Phytophthora infestans, the effects of different media, temperature, pH and light on the growth of bacteria and sporangium were studied. The results showed that both V8 juice and carrot medium were suitable for mycelial growth, while V8 juice, corn flour and tomato medium were suitable for producing sporangium for bacteria, especially for tomato agar medium. Bacteria can grow in the range of 10 ~ 35 ℃, the optimum temperature range is 24 ~ 32 ℃. The optimum pH value is 5 ~ 8, when the pH is 7 ~ 8 slightly alkaline, mycelium grows fastest. Light conditions on mycelial growth is not obvious, but significantly promote the production of sporangia.