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目的:通过体内外实验研究双黄调脂方的降脂作用,探讨其降脂机制。方法:昆明种小鼠随机分为正常组、模型组、阳性药组(血脂康,0.6 g·kg~(-1))和双黄调脂高、低剂量组(22.5,7.5 g·kg~(-1)),观察双黄调脂方对Trtion-WR1339诱导的高血脂模型的影响。基于整体实验,体外实验观察了双黄调脂主要有效成分姜黄素、黄连素、葛根素对油酸(oleic acid,OA)诱导的HepG2细胞模型胆固醇(CHO),甘油三酯(TG),3-羟基-3-甲基戊二酰CoA(HMG-CoA)还原酶,高密度脂蛋白胆固醇(HDLC),低密度脂蛋白受体(LDL-R),胆固醇7α-羟化酶(CYP7A1)和脂蛋白脂酶(LPL)含量的影响,探讨其可能降脂途径。结果:与正常组比较,模型组小鼠血清CHO和TG含量显著升高(P<0.01);与模型组比较,双黄调脂方高剂量组能显著降低模型小鼠血清的CHO,TG水平(P<0.05,P<0.01)。体外实验中,与空白组比较,模型组细胞CHO,TG和HMG-CoA还原酶含量显著升高(P<0.05,P<0.01),HDL-C,LDL-R,CYP7A1和LPL含量显著降低(P<0.05);与模型组比较,姜黄素高剂量组(10 mg·L~(-1))能显著升高模型细胞HDL-C,LDL-R和LPL含量(P<0.05,P<0.01),降低TG和HMG-CoA含量(P<0.05,P<0.01);黄连素高剂量组(40 mg·L~(-1))能显著升高模型细胞CHO,HDL-C,LDL-R和CYP7A1含量(P<0.05,P<0.01);葛根素高剂量组(300 mg·L~(-1))能显著升高模型细胞HDL-C,LDL-R,CYP7A1和LPL含量(P<0.05,P<0.01),降低CHO,TG和HMG-CoA含量(P<0.05,P<0.01)。结论:双黄调脂方可通过抑制内源性胆固醇合成、促进胆固醇逆转运(RCT)途径和低密度脂蛋白(LDL)受体途径、促进胆固醇转化以及促进甘油三酯降解等多个途径来发挥降脂作用。
Objective: To study the lipid-lowering effect of Shuanghuang Tiaozhi prescription in vitro and in vivo, and to explore the mechanism of lipid-lowering. Methods: Kunming mice were randomly divided into normal group, model group, positive group (Xuezhikang, 0.6 g · kg -1) and Shuanghuanglipian high and low dose groups (22.5 g · kg ~ (-1)) to observe the Shuanghuang Tiaozhi on Trtion-WR1339-induced hyperlipidemia model. Based on the whole experiment, we observed the effects of curcumin, berberine and puerarin on HepG2 cells induced by oleic acid (OA), cholesterol (CHO), triglyceride (TG) HMG-CoA reductase, high density lipoprotein cholesterol (HDLC), low density lipoprotein receptor (LDL-R), cholesterol 7α-hydroxylase (CYP7A1) and Lipoprotein lipase (LPL) content, to explore its possible lipid-lowering pathways. Results: Compared with the normal group, the levels of serum CHO and TG in the model group were significantly increased (P <0.01). Compared with the model group, the Shuanghuangliaozhifang high-dose group could significantly reduce the levels of CHO and TG in the model mice (P <0.05, P <0.01). Compared with the blank group, the contents of CHO, TG and HMG-CoA reductase in the model group were significantly increased (P <0.05, P <0.01) and the levels of HDL-C, LDL-R, CYP7A1 and LPL were significantly decreased (P <0.05). Compared with the model group, high dose of curcumin (10 mg · L -1) significantly increased HDL-C, LDL-R and LPL (P <0.05, P <0.01). High doses of berberine (40 mg · L -1) significantly increased the contents of CHO, HDL-C and LDL-R (P <0.05, P <0.01). High dose Puerarin (300 mg · L -1) significantly increased the content of HDL-C, LDL-R, CYP7A1 and LPL in model cells (P < 0.05, P <0.01), and decreased the content of CHO, TG and HMG-CoA (P <0.05, P <0.01). Conclusion: Shuanghuang Tiaozhi can inhibit the synthesis of endogenous cholesterol, promote the reverse cholesterol transport (RCT) pathway and the low density lipoprotein (LDL) receptor pathway, promote cholesterol conversion and promote the degradation of triglyceride and other ways to Play lipid-lowering effect.