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目的建立止咳丸中(-)薄荷酮、薄荷脑含量测定方法。方法色谱柱HP-FFAP毛细管柱(25m×0.2mm,0.3μm);程序升温:初始温度90℃,保持2min,以6℃·min-1的速率升至150℃,再以30℃·min-1的速率升温至220℃,保持4min;载气为高纯氮,氢火焰离子化检测器(FID),进样口温度:230℃;检测器温度:250℃;分流比为10:1,进样量1.0μL。结果 (-)薄荷酮在0.04486～0.8972mg·mL-1(r=0.9997)内平均回收率为98.12%,RSD=0.86%;薄荷脑在0.06488～0.9732mg·mL-1(r=0.9998)内平均回收率为100.91%,RSD=1.16%,均呈良好线性关系。结论本法快速、简便、准确、可靠,可用于控制止咳丸的质量。
Objective To establish a method for determination of (-) menthone and menthol in Zhike Wan. Methods The column was HP-FFAP capillary column (25m × 0.2mm, 0.3μm). The temperature was programmed at 90 ℃ for 2min and then increased to 150 ℃ at 6 ℃ · min-1. 1 at a rate of 220 ° C for 4 min. The carrier gas was high purity nitrogen, hydrogen flame ionization detector (FID). The inlet temperature was 230 ° C. The detector temperature was 250 ° C. The split ratio was 10: Injection volume 1.0μL. Results The mean recoveries of (-) menthone in the range of 0.04486 ~ 0.8972 mg · mL-1 (r = 0.9997) were 98.12% and RSD = 0.86%, respectively. Menthol was in the range of 0.06488 ~ 0.9732 mg · mL- The average recovery was 100.91%, RSD = 1.16%, showing a good linear relationship. Conclusion This method is rapid, simple, accurate and reliable and can be used to control the quality of cough pill.