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为了观察表达胶质细胞系源性神经营养因子 (GDNF)的基因工程细胞在脑内移植后对帕金森病大鼠的治疗作用 ,将 6-羟基多巴胺脑内定位单侧 (损伤侧 )注射制备大鼠帕金森病模型。随机将动物分为实验组 (2 0只 )和对照组 (15只 ) ,分别植入GDNF和 Lac Z转基因的原代培养大鼠成纤维细胞于损伤侧纹状体内 ,动态观察动物单侧旋转行为以及多巴胺及其代谢产物 3 ,4-二羟苯乙酸和高香草酸含量的变化。结果表明 :实验组帕金森病大鼠单侧旋转行为明显减少 ,为移植前旋转圈数的 (64 .8± 5 .8) % (P<0 .0 5 ) ;其纹状体内多巴胺含量显著提高 ,恢复至移植前水平的 (14 .5± 2 .2 ) % (P<0 .0 5 ) ,而对照组仅为 (1.92± 0 .15 ) % ;转基因细胞在脑内可存活 1年以上。本研究结果提示 GDNF基因治疗对大鼠帕金森病具有潜在的临床应用价值。
In order to observe the therapeutic effect of genetically engineered cells expressing glial cell line-derived neurotrophic factor (GDNF) on Parkinson’s disease rats after intracerebral transplantation, 6-hydroxydopamine intracerebral unilateral (lesion side) injection was prepared Parkinson’s disease model in rats. Animals were randomly divided into experimental group (20 rats) and control group (15 rats). Primary cultured rat fibroblasts implanted with GDNF and LacZ transgene were implanted into the injured lateral striatum. Animals were unilaterally rotated Behavior and dopamine and its metabolites 3,4-dihydroxyphenylacetic acid and high vanillic acid content changes. The results showed that the unilateral rotation of rats in Parkinson’s disease group was significantly decreased, which was (64.8 ± 5.8)% (P <0.05) before transplantation. The content of dopamine in striatum was significantly Increased to (14.5 ± 2.2)% (P <0.05) before transplantation, but only 1.92 ± 0.15% in control group. Transgenic cells survived in the brain for 1 year the above. The results suggest that GDNF gene therapy has potential clinical value in the treatment of Parkinson’s disease in rats.