本文介绍用高效液相色谱法测定罗布麻浸膏中懈皮素。使用岛津 LC—2型高压液相色谱仪,YWG—C_(18)H_(35)填充柱(25cm×4.6mm i.d.)。以1.5%磷酸的50%甲醇混合液作流动相,紫外254nm 检测,用内标法定量,内标物为β—萘酚。测定结果,罗布麻浸膏中檞皮素的含量平均为0.17%,相对标准偏差3.2%(8次测定)。本法简便,快速,重现性好。 This article describes the determination of quercetin in apocynum extract by high performance liquid chromatography. A Shimadzu LC-2 type high pressure liquid chromatograph was used with a YWG-C_(18)H_(35) packed column (25 cm x 4.6 mm i.d.). The mobile phase was a mixture of 1.5% phosphoric acid in 50% methanol, detected by UV at 254 nm, quantified by internal standard method, and the internal standard was β-naphthol. As a result of the measurement, the content of quercetin in the apocynum extract was 0.17% on average with a relative standard deviation of 3.2% (8 determinations). This method is simple, rapid, and reproducible.