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目的:观察孕鼠接触葡萄球菌肠毒素B(SEB)对其子代新生鼠胸腺及外周血TCR Vβ8~+T细胞的影响。方法:在妊娠16 d时给予SD大鼠尾静脉注射15μg SEB(SEB组),同时设立注射磷酸盐缓冲溶液对照组。孕鼠自然分娩后获取出生后0~5 d的子代新生鼠胸腺及外周血,流式细胞仪检测其TCR Vβ8~+T细胞比例。结果:与对照组比较,SEB组出生后0~3 d的新生鼠胸腺中CD4~+Vβ8~+T细胞比例均减少(P<0.05~P<0.01),出生后4~5 d 2组差异均无统计学意义(P>0.05);出生后0~5 d,SEB组胸腺中CD8~+Vβ8~+T细胞比例均低于对照组(P<0.05~P<0.01);出生后0~5 d,SEB组CD4~+Vβ8~+及CD8~+Vβ8~+T细胞的绝对数均较对照组明显减少(P<0.01)。新生鼠出生后0~5 d,SEB组外周血CD4~+Vβ8~+T细胞比例均较对照组明显减少(P<0.01);CD8~+Vβ8~+T细胞比例亦均较对照组减少(P<0.05~P<0.01);出生后0~5 d,SEB组外周血CD4~+Vβ8~+及CD8~+Vβ8~+T细胞绝对数均较对照组明显减少(P<0.01)。结论:妊娠期大鼠接触SEB可减少子代新生鼠胸腺及外周血TCR Vβ8~+T细胞,并保留至成年期。
Objective: To observe the effect of exposure to staphylococcal enterotoxin B (SEB) on TCR Vβ8 ~ + T cells in thymus and peripheral blood of offspring neonatal rats. Methods: SD rats were injected intravenously with 15 μg of SEB (SEB group) on the 16th day of gestation, and the control group was injected with phosphate buffered saline. Pregnant mice were naturally born after delivery 0 ~ 5 d offspring neonatal thymus and peripheral blood, flow cytometry TCR Vβ8 ~ T cells ratio. Results: Compared with the control group, the percentage of CD4 ~ + Vβ8 ~ + T cells in the thymus of neonatal rats 0-3 days after birth in SEB group decreased (P <0.05 ~ P <0.01) (P <0.05 ~ P <0.01). From 0 to 5 days after birth, the proportion of CD8 ~ + Vβ8 ~ + T cells in thymus of SEB group was significantly lower than that of control group At 5 days, the absolute numbers of CD4 ~ + Vβ8 ~ + and CD8 ~ + Vβ8 ~ + T cells in SEB group were significantly decreased compared with those in control group (P <0.01). Compared with control group, the percentage of CD4 ~ + Vβ8 ~ + T cells in peripheral blood of SEB group was significantly decreased (P <0.01) at 0 ~ 5 d after birth, and the proportion of CD8 ~ + Vβ8 ~ + T cells in control group was also decreased P <0.05 ~ P <0.01). From 0 to 5 days after birth, the absolute numbers of CD4 ~ + Vβ8 ~ + and CD8 ~ + Vβ8 ~ + T cells in SEB group were significantly lower than those in control group (P <0.01). Conclusion: Exposure of pregnant rats to SEB can reduce the TCR Vβ8 ~ + T cells in neonatal thymus and peripheral blood of offspring, and keep it to adulthood.