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本文采用CM-Sephadex C-50柱层析分离金环蛇原毒,得16个峰,其中组份Ⅰ有促进兔血小板聚集的作用,其聚集曲线呈两个聚集波,原毒及组份Ⅶ～ⅩⅣ对ADP诱导的血小板聚集有抑制作用,其中组份Ⅺ经DEAE-Sephadex A-50柱层析纯化,经聚丙烯酰胺圆盘电泳鉴定为单一区带,组份Ⅺ可使小鸡颈二腹肌产生挛缩,且抑制离体蛙心收缩,该组份没有ADP酶及5′-核苷酸酶活力,但有磷脂酶A_2活力,原毒及组份Ⅺ的LD_(50)(小鼠ip)分别为1.5±s 0.2及8.0±s 1.3 mg·kg~(-1)(95％可信限),组份Ⅺ不引起富血小板血浆中乳酸脱氢酶含量升高,对ADP,花生四烯酸,A23187及凝血酶诱导的血小板聚集均有抑制作用,其IC_(50)分别为:68.8,10.0,10.1,6.3μg·ml~(-1),但对血小板5羟色胺的释放无明显影响,其作用机理需进一步研究。
In this paper, CM-Sephadex C-50 column chromatography was used to isolate Genus Horsetail, 16 peaks, of which component I promote platelet aggregation in rabbits, the aggregation curve showed two aggregated waves, the original virus and the composition of Ⅶ ~ Ⅹ Ⅳ on ADP-induced platelet aggregation inhibition, which component XI by DEAE-Sephadex A-50 column purification by polyacrylamide disk electrophoresis identified as a single zone, component XI can make chick two Abdominal muscle produce contracture, and inhibit the contraction of frog in vitro, this component is not ADP enzyme and 5’-nucleotidase activity, but phospholipase A_2 activity, the original toxicity and component Ⅺ LD_ (50) (mouse ip) were 1.5 ± s 0.2 and 8.0 ± s 1.3 mg · kg -1 (95% confidence limits) respectively. However, component XI did not cause an increase of lactate dehydrogenase in platelet-rich plasma. Tetraenoic acid, A23187 and thrombin-induced platelet aggregation were inhibited, the IC 50 values were 68.8, 10.0, 10.1 and 6.3 μg · ml -1, respectively, but no significant effect on platelet serotonin release Influence, its mechanism of action needs further study.