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目的:研究Ang-1、Ang-2和Tie-2及VEGF蛋白在三阴性乳腺癌(TNBC)组织中的表达及其与MVD的关系,并分析其与TNBC临床病理学特征及其预后的关系。方法:应用免疫组化SP法对45例TNBC组织中Ang-1、Ang-2和Tie-2及VEGF蛋白表达进行检测,并用同样方法检测45例TNBC组织中CD34的表达以评估MVD。结果:TNBC肿瘤组织中Ang-1、Ang-2和Tie-2及VEGF蛋白表达率分别为31.1%(14/45)、57.8%(26/45)、44.4%(20/45)和66.7%(30/45)。Ang-2蛋白表达与Tie-2蛋白表达呈正相关(r=0.312,P=0.037),Ang-2蛋白表达与VEGF蛋白表达呈正相关(r=0.350,P=0.018)。MVD在Ang-2蛋白阳性表达组显著高于阴性表达组(t=-9.110,P=0.000);MVD在VEGF蛋白阳性表达组显著高于阴性表达组(t=-0.889,P=0.000)。Ang-1、Ang-2、和Tie-2及VEGF蛋白表达与年龄、绝经状态、病理分期、肿瘤大小、淋巴结转移均无相关性(P均>0.05)。TNBC术后生存时间与Ang-1、Ang-2、Tie-2或VEGF蛋白表达均无关,P>0.05。结论:Ang-2与VEGF蛋白有协同作用,促进TNBC血管形成。Ang-1、Ang-2和Tie-2及VEGF蛋白表达与TNBC临床病理因素和术后生存无相关性,均不能作为TNBC患者的预后预测指标。
Objective: To investigate the expression of Ang-1, Ang-2, Tie-2 and VEGF in triple negative breast cancer (TNBC) and its relationship with MVD, and to analyze its relationship with clinicopathological features and prognosis of TNBC . Methods: The expressions of Ang-1, Ang-2, Tie-2 and VEGF in 45 TNBC tissues were detected by immunohistochemical SP method. The expression of CD34 in 45 TNBC tissues was detected by the same method to evaluate MVD. Results: The expressions of Ang-1, Ang-2, Tie-2 and VEGF in TNBC were 31.1% (14/45), 57.8% (26/45), 44.4% (30/45). Ang-2 protein expression was positively correlated with Tie-2 protein expression (r = 0.312, P = 0.037). Ang-2 protein expression was positively correlated with VEGF protein expression (r = 0.350, P = 0.018). The MVD in Ang-2 positive group was significantly higher than that in negative group (t = -9.110, P = 0.000). The positive rate of MVD in VEGF positive group was significantly higher than that in negative group (t = -0.889, P = 0.000). Ang-1, Ang-2, Tie-2 and VEGF protein expression had no correlation with age, menopause, pathological stage, tumor size and lymph node metastasis (all P> 0.05). Survival time of TNBC had no correlation with Ang-1, Ang-2, Tie-2 or VEGF protein expression (P> 0.05). Conclusion: Ang-2 and VEGF proteins have a synergistic effect and promote TNBC angiogenesis. The expressions of Ang-1, Ang-2, Tie-2 and VEGF were not correlated with TNBC clinical pathological factors and postoperative survival, and could not be used as predictors of prognosis in patients with TNBC.