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在急性胃炎,良性胃溃疡或十二指肠溃疡患者胃活检标本培养中常有胃窦弯曲杆菌(Campylo-bacter Pyloridis)生长。此菌生长较为缓慢,因而采用培养这种方法进行检查,对胃窦弯曲杆菌感染的诊断将会耽搁。如用HE,Giemsa 或Warthin-Starry 染色进行组织学检查,在上皮表面也多能见到胃窦弯曲杆菌。Warthin-Starry 银染操作较为繁琐,但易检出此菌,而HE 和Giemsa 染色则操作容易,但要花费很多时间才能发现细菌。吖啶橙是一种荧光色素,可与核酸结合,临床上用来检测微生物。作者发现对福尔马林固定,石蜡包埋的组织学切片,可采用吖啶橙使胃窦弯曲杆菌着色。可快速检测胃窦弯曲杆菌,具体方法如下:
Campylobacter pyloridis is often grown in gastric biopsy specimens of patients with acute gastritis, benign gastric ulcer, or duodenal ulcer. This bacteria grow more slowly, so using this method of culture for examination, the diagnosis of Campylobacter infection will be delayed. Histological examination, such as HE, Giemsa, or Warthin-Starry staining, can also be found more often on the surface of the epithelium. Warthin-Starry silver staining is cumbersome, but it is easy to detect, whereas HE and Giemsa stains are easy to handle, but it takes a lot of time to spot bacteria. Acridine orange is a kind of fluorescent pigment, which can be combined with nucleic acid and used clinically to detect microorganisms. The authors found that for formalin-fixed, paraffin-embedded histological sections, A. campylobacter can be stained with acridine orange. Campylobacter can be quickly detected, the specific method is as follows: