以4个牡丹品种、8个芍药品种为亲本,进行了杂交亲和性研究。结果表明:牡丹“彩绘×冠群芳”和芍药“粉玉奴×红玛瑙”组内杂交具有较高的亲和性;以芍药“粉玉奴”为母本,分别以牡丹“彩绘”、“粉中冠”为父本杂交获得了远缘杂交种子,并用ISSR分子标记对牡丹芍药远缘杂交后代进行真实性鉴定,2个子代确定为芍药和牡丹的远缘杂交后代,均属偏母型。扩增结果中出现了父母本都不具有的谱带,说明子代不仅遗传了亲本的特征而且突变产生亲本所不具备的新性状。 Four peony varieties and eight peony cultivars were used as parents to study the hybridization affinity. The results showed that the hybrids of Peony “Painted Xuanqunfang ” and Peony “Fengyu Nu Onyx” had high affinity in hybrids. Using peony “Fengyu slaves ” as female parent, Peony “painted ”, “pink crown ” as the male parent hybrid access to the distant hybrid seed and ISSR molecular markers on Peony peony distant hybridization progeny were identified, two offspring identified as peony and peony Distant hybrids offspring, are partial mother type. The results of the amplification showed that the parents did not have the band, indicating that not only the offspring inheritance of the characteristics of the parent and the mutation produces new traits that parents do not have.