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用质谱法研究了人参皂苷Rb1和Rd与细胞色素c的非共价相互作用,证明主-客体之间形成了多种化学计量比的复合物,用直接计算的方法得出人参皂苷Rb1和Rd与细胞色素c形成的非共价复合物的各级解离常数KD,用竞争体系验证了计算结果,二者结果相互吻合.
The non-covalent interaction of ginsenosides Rb1 and Rd with cytochrome c was studied by mass spectrometry. It was proved that a variety of stoichiometric complexes were formed between the host and guest. The ginsenosides Rb1 and Rd were calculated by direct calculation. The dissociation constants KD of the non-covalent complexes formed with cytochrome c were verified by the competitive system. The results were consistent with each other.