西瓜枯萎病菌在正常生长条件下,产生大型分生孢子和厚坦孢子的速度慢,且数量少。促使其产生孢子并将菌种保存好.曾是我们研究中急待解决的问题。现把多年摸索出的简易解决方法简介于下。 一.促使大型分生孢子产生的方法 将纯菌种接种在PDA平板培养基上,在25℃下培养5天左右,待菌落生长繁茂后,在无菌操作的条件下,将菌落连同培养基一起铲放在盛有无菌水的灭菌培养皿中。无菌水仅仅淹没培养基及菌落的基部,使菌落的上部露于水面。盖上皿盖,放在25℃的温度下培养3-7天后,在双目解剖镜下可以看到菌落中的菌丝上长有很多分生孢子梗及其上的大型分生孢子。 二.促使厚坦孢子产生的方法 将生长繁茂的菌落连同培养基一起铲放在装有无菌水的灭菌培养基皿中(其作法同上)。使无菌水将培养基和菌落全部淹没。盖上皿盖,放在25℃下培养5天左右,在双目解剖镜下,可以看到菌丝的中部和预部形成有大量的厚坦孢子。 三.用干燥土壤保存菌种的方法 Watermelon Fusarium wilt in normal growth conditions, the production of large conidia and thick conidia slow, and a small number. Which caused spores to be produced and kept the strain well, has been an urgent problem in our research. Now find out the simple solution for many years briefed on the next. A method to promote the formation of large conidia Pure species inoculated on PDA plate medium, cultured at 25 ℃ for about 5 days until the colonies grow lush, in aseptic conditions, the colonies together with the medium Shovel together in a sterile petri dish containing sterile water. Sterile water just submerged the bottom of the culture medium and colonies so that the upper part of the colonies was exposed to the water. Cover the dish cover and incubate for 3-7 days at a temperature of 25 ℃. There are many conidiophores and large conidia on the mycelium in the colony under the binocular dissection microscope. Method for Promoting the Production of Protocarcinoma The growing colonies are shoveled along with the culture medium in a sterilized culture medium containing sterilized water (as described above). Sterile water completely submerges the medium and colonies. Cover the dish cover and put it in culture at 25 ℃ for about 5 days. Under the binocular dissection microscope, we can see that there are a large number of thick conidia in the middle part and pre-part of mycelium. Third, use dry soil to save bacteria method