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目的:观察柴胡皂甙D(saikosaponin-d,SSd)对二乙基亚硝胺(diethylnitrosamine,DEN)致大鼠肝癌免疫功能的影响,并探讨其与SSd抗肝肿瘤作用的关系.方法:清洁级♂SD大鼠90只,平均体质量248.18±12.32g,随机分为5组:模型组(n=20),对照组(n=10)及SSd大、中、小剂量治疗组(均n=20).除对照组给予等量生理盐水灌胃外,其余各组大鼠均给予2mg/LDEN灌胃,按体质量10mg/kg给药,每周5次,同时各治疗组每天给予不同浓度SSd(2.0、1.5、1.0mg/kg)腹腔注射,至16wk停药,麻醉后处死大鼠.HE染色观察实验大鼠肝组织病理学结构的改变,应用流式细胞仪测定大鼠外周血T淋巴细胞亚群(CD3+CD4+%、CD3+CD8+%及CD3+CD4+/CD3+CD8+比值).结果:SSd各治疗组大鼠癌结节数及灶的大小均小于模型组.镜下单纯造模组癌细胞呈多形性,异形性明显,Edmondson分级多数属于Ⅲ级;相反,SSd各干预组癌细胞分化程度高,异型性较低,分级多为Ⅰ-Ⅱ级;肝癌模型组与正常对照组T淋巴细胞亚群比较CD4+、CD4+/CD8+明显下降,CD8+上升,差异有统计学意义(33.56%±4.16%vs45.50%±4.03%;1.06±0.56vs1.93±0.28;30.62%±3.65%vs22.88%±3.15%;均P<0.05);而SSd各组与肝癌模型组T淋巴细胞亚群比较CD4+、CD4+/CD8+明显回升,CD8+下降,差异有统计学意义,尤其以SSd大剂量组明显(39.06%±3.98%vs33.56%±4.16%;1.55±0.29vs1.06±0.56;18.99%±3.09%vs30.62%±3.65%;均P<0.05).结论:SSd对DEN诱发大鼠肝癌形成有一定的免疫保护作用.
Objective: To observe the effect of saikosaponin-d (SSd) on the immune function of hepatocarcinoma induced by diethylnitrosamine (DEN) in rats and its relationship with the anti-hepatoma effect of SSd.Methods: 90 male SD rats with an average body weight of 248.18 ± 12.32g were randomly divided into 5 groups: model group (n = 20), control group (n = 10) and SSd large, medium and small dose treatment groups = 20) .In addition to the control group given the same amount of saline gavage, the other groups were given 2mg / LDEN gavage, according to the body weight of 10mg / kg administration, 5 times a week, while each treatment group given different daily The rats were sacrificed at 16wk after treatment with SSd (2.0,1.5,1.0mg / kg), and the rats were killed after anesthesia.HE staining was used to observe the pathological changes of liver tissue in experimental rats, and the peripheral blood of rats T lymphocyte subsets (CD3 + CD4 +%, CD3 + CD8 +% and CD3 + CD4 + / CD3 + CD8 + ratio) .Results: The number of cancerous nodules and the size of lesion in each treatment group were less than those in model group The cancerous cells of the model group were pleomorphic with obvious atypia. Most Edmondson grade belonged to grade Ⅲ. On the contrary, the SSd of each intervention group had high degree of differentiation and low atypia, The levels of CD4 +, CD4 + / CD8 + and CD8 + in T lymphocyte subsets of model group and normal control group were significantly decreased (33.56% ± 4.16% vs45.50% ± 4.03%; 1.06 ± 0.56vs1.93 ± 0.28 ; 30.62% ± 3.65% vs22.88% ± 3.15% respectively; all P <0.05); however, the levels of CD4 +, CD4 + / CD8 + of CD4 + and CD4 + / CD8 + in SSd groups were significantly increased (39.06% ± 3.98% vs33.56% ± 4.16%; 1.55 ± 0.29vs1.06 ± 0.56; 18.99% ± 3.09% vs30.62% ± 3.65% respectively; all P <0.05), especially in SSd high dose group. Conclusion: SSd can protect DEN-induced hepatocarcinogenesis in rats.