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建立乳酸环丙沙星泊洛沙姆原位凝胶中乳酸环丙沙星含量测定的高效液相色谱法。以流动相为参比溶液,在波长200～400 nm段用紫外分光光度计进行光谱扫描以选择乳酸环丙沙星的入射波长;用Extreme C18柱(5μm,25 cm×4.6 mm)为色谱柱,流动相为0.025 mol/L磷酸溶液(三乙胺调节pH至3)-乙腈(87:13),流速为1.0 mL/min,检测波长为λmax=279 nm,进样量为50μL,采用峰面积外标标准曲线法计算含量。结果表明:乳酸环丙沙星的最大吸收波长为279 nm,峰宽较窄;乳酸环丙沙星检测质量浓度线性范围为1.25～40μg/mL(r=1),平均回收率99.50%(相对标准偏差=1.67%,n=9)。本法操作简便,快速,结果准确可靠,重复性较好,可用于测定乳酸环丙沙星原位凝胶中乳酸环丙沙星的含量。
To establish a HPLC method for the determination of ciprofloxacin lactate in ciprofloxacin lactate in situ gel. The mobile phase was used as the reference solution, and the wavelength of 200-400 nm was scanned by ultraviolet spectrophotometer to select the incident wavelength of ciprofloxacin lactate. The column with Extreme C18 (5 μm, 25 cm × 4.6 mm) . The mobile phase consisted of 0.025 mol / L phosphoric acid (adjusted to pH 3 with triethylamine) -acetonitrile (87:13), the flow rate was 1.0 mL / min, the detection wavelength was λmax = 279 nm and the injection volume was 50 μL. Area standard method of calculating the standard external standard. The results showed that the maximum absorption wavelength of ciprofloxacin lactate was 279 nm and the peak width was narrow. The linear range of the concentration of ciprofloxacin lactate was 1.25-40 μg / mL (r = 1) with the average recovery of 99.50% Standard deviation = 1.67%, n = 9). The method is simple, rapid, accurate, reliable and reproducible. It can be used to determine the content of ciprofloxacin lactate in situ gel of ciprofloxacin lactate.