The association of gluckinase (GCK) gene with type 2 (non-insulin-dependent) diabetes mellitus was investigated in 168 Chinese subjects (85 unrelated type 2 diabetics and 83 non-iabetic controls). The microsatellite polymorphism marker, GCK-5’, was amplified with polymerase chain reaction. Four alleles were observed in Chinese population with length varying from 137bp to 143bp and the most common one being the 139bp allele 3. In comparison with non-iabetics, allele 4 was significantly increased in type 2 diabetes (10% versus 38, respectively; X2 = 6.773, P = 0.009); genotype 44 and 4X (X de notes any allele other than allele 4) were significantly increased in type 2 diabetes (16% versus 6% respectively; X2 = 6.439. P = 0.011). The frequency difference was also shown in overweight / obese subgroup comparison (X2 =7.718, P = 0.021), but not in lean / normal weight subgroup comparison. No differences of age of onset and frequency of positive family history were observed between type 2 diabetic patients wit The association of glukginase (GCK) gene with type 2 (non-insulin-dependent) diabetes mellitus was investigated in 168 Chinese subjects (85 unrelated type 2 diabetics and 83 non-iabetic controls). The microsatellite polymorphism marker, GCK- was amplified with polymerase chain reaction. Four alleles were observed in Chinese population with length varying from 137bp to 143bp and the most common one being the 139bp allele 3. In comparison, non-iabetics, allele 4 was significantly increased in type 2 diabetes (10 % versus 38, respectively; X2 = 6.773, P = 0.009); genotype 44 and 4X (X de notes any allele other than allele 4) were significantly increased in type 2 diabetes (16% versus 6% respectively; = 0.011). The differences were also shown in overweight / obese subgroup comparison (X2 = 7.718, P = 0.021), but not in lean / normal weight subgroup comparison. No differences of age of onset and frequency of positive family history were observed between type 2 dia betic patients wit