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目的 :探讨 HBV未分离成功的情况下研究 HBV父儿传播途径的可行方法。方法 :以巢式 PCR检测 HBV DNA与序列分析的方法比较父亲与胎儿所携 HBV S区 nt45 1～ 6 6 0段 ,C区 nt2 0 2 2～ 2 32 1段序列的一致性。结果 :父儿间两段序列同源性在 98%～ 10 0 %之间。 S区检出 491、 494、 5 30、 5 46、 5 81位变异致使 113、 114、 12 6、 131、 143位氨基酸替代。结论 :巢式 PCR与序列分析方法是目前病原体未分离成功的情况下研究传播途径的可行方法。
Objective: To investigate the possible method of studying the transmission of HBV in the case of HBV without the successful isolation of HBV. Methods: The nested PCR detection of HBV DNA and sequence analysis was used to compare the consistency of nt2 0 2 2 ~ 2 32 1 sequences between nt45 1 ~ 6 6 0 and nt C in HBV S region between father and fetus. Results: The homology of two sequences between father was between 98% ~ 100%. The mutation of 491, 494, 5 30, 5 46, 5 81 in the S region resulted in amino acid substitutions of 113, 114, 12 6, 131 and 143. Conclusion: The method of nested PCR and sequence analysis is a feasible method to study the route of transmission when the pathogen is not successfully isolated.