研究局部复发和原发肿瘤的二次发展(SPT)是影响口腔鳞状细胞癌(OSCC)患者生存率的重要因素。这篇研究是为了评估研究者们提出的癌变区域理念:与原发性肿瘤相邻的正常组织存在癌前病变,可导致局部复发和SPTs发展。为了验证是否存在癌变区域的理念,研究者们应用全外显子和靶向超深测序技术检测5个新鲜冻存的样本。样本来源于III级OSCC患者的 To study the local recurrence and the secondary development of primary tumors (SPT) is an important factor affecting the survival rate of patients with oral squamous cell carcinoma (OSCC). The purpose of this study was to evaluate the concept of cancerous regions proposed by researchers: precancerous lesions exist in normal tissues adjacent to the primary tumor, leading to local recurrence and development of SPTs. To validate the concept of a cancerous region, the researchers tested five freshly frozen samples using whole exon and targeted ultra-deep sequencing. The samples come from patients with grade III OSCC