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鉴于耳蜗缺少神经支配的耳聋病人不适用人工耳蜗,为此,目前的研究正朝向中枢听觉神经的赝复物方面发展。本文旨在用中枢听觉通路的糖代谢来评价这种发计的可行性。作者选用24只豚鼠,共分成五组,埋植组豚鼠均在无菌操作下,经枕后开颅,置入经76.2μm 的铂铱双电极,通过电刺激诱发出中潜伏期反应(EMLR),来判断电极是否进入耳蜗核(CN)。另给豚鼠腹腔注射以~(14)C 标记的2-脱氧葡萄糖(2-DG),经过1小时的结合,处杀动物,迅速取出脑组织,置入干冰中冷冻,制作20μm 的切片,在低温恒温箱中干燥,将这些带放射活性的切片使 X-线胶片曝光、和标准的
In view of the cochlear cochlea lack of innervation of the deaf patients do not apply cochlear, for which, the current study is toward the central auditory nerve prostheses development. This article aims to assess the feasibility of this finding using the glucose metabolism of the central auditory pathway. The authors selected 24 guinea pigs, divided into five groups, the guinea pigs were implanted under aseptic conditions, after occipital craniotomy, Placed by 76.2μm platinum iridium double electrode, induced by electrical stimulation in the latent period response (EMLR) , To determine whether the electrode into the cochlear nucleus (CN). The guinea pigs were intraperitoneally injected with ~ (14) C-labeled 2-deoxyglucose (2-DG), and the animals were killed after 1 hour of binding. The brains were quickly removed and frozen in dry ice to prepare 20 μm sections. X-ray film was exposed to these radioactive sections by drying in a cryostat, and the standard