论文部分内容阅读
目的 观察钝顶螺旋藻多糖 (Spirulinaphatensispolysaccharide ,SPP)对辐射损伤小鼠造血功能的影响。方法 小鼠5Gy全身照射后 5、10、15d分别取材 ,作骨髓粒单系、红系造血祖细胞培养 ,造血干细胞集落测定 ,以及外周血常规计数等。SPP用量为 12 5mg·kg 1 ·d 1 ,ip,伤后连续注射 6d。结果 辐射损伤后骨髓有核细胞数、粒单系CFU GM、红系CFU E、BFU E及造血干细胞CFU S集落数均显著低于正常对照组 (P <0 .0 1) ,但应用SPP后 ,上述各项指标数值 ,均比同伤情的不用药组显著提高 ,尤以伤后10d恢复最快 (P <0 .0 1)。结论 SPP对辐射损伤有明显保护和促进造血恢复的作用。
Objective To observe the effect of Spirulina aptonensis polysaccharide (SPP) on hematopoietic function in radiation-injured mice. METHODS: Whole body irradiation of 5Gy mice was performed on 5th, 10th, and 15th day respectively for culture of bone marrow monophyletic, erythroid hematopoietic progenitor cells, hematopoietic stem cell colony determination, and peripheral blood routine counting. The dose of SPP was 12 5mg·kg 1 ·d 1 , ip, and was injected continuously for 6 days after injury. Results The number of bone marrow nucleated cells, CFU GM, erythroid CFU E, BFU E and hematopoietic stem cells CFU S colony counts after radiation injury were significantly lower than those in the normal control group (P < 0.01), but after SPP The values of the above indicators were significantly higher than those of the non-drug group with the same injury, especially the recovery was the fastest at 10 days after injury (P < 0.01). Conclusion SPP has significant protection against radiation damage and promotes hematopoietic recovery.