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■癌患者发生免疫系统活性低下。为搞清免疫抑制物质的来源,作者在实验研究中应用豚鼠的10系肿瘤,明确了免疫抑制物质是存在于肿瘤细胞表面的蛋白质。本文报告肿瘤细胞表面分离的成分对动物巨噬细胞的作用及对动物血液成分的影响。豚鼠10系肝细胞瘤细胞经豚鼠腹腔内接种传代,取腹水1500rpm 离心20分钟,把除去肿瘤细胞后的上清用于实验。用水提取法提取的肿瘤细胞表面成分(WEM)冰冻干燥备用。将此 WEM1周内连日给 BALB/c 雄性小鼠及裸鼠腹腔注射,每只500μg,第8日杀死动物采血。对照组给生理盐水。用自动血球计数装置进行血液成分分析,另外,研究了 WEM 对 BALB/c 小鼠腹腔巨噬细胞的作用。小鼠腹腔内注射硫乙
■ Low incidence of immune system activity in cancer patients. In order to understand the source of immunosuppressive substances, the authors used guinea pig 10-line tumors in experimental studies, and it was clarified that immunosuppressive substances are proteins present on the surface of tumor cells. This article reports the effects of the components of tumor cell surface separation on macrophages in animals and the effects on animal blood components. Guinea pig line 10 hepatoma cells were passaged intraperitoneally to guinea pigs, and ascites was centrifuged at 1500 rpm for 20 minutes. The supernatant after removal of tumor cells was used for the experiment. The surface of the tumor cells (WEM) extracted with water extraction was freeze-dried for use. This WEM was intraperitoneally injected to BALB/c male mice and nude mice intraperitoneally every week for 1 week, 500 μg each, and animals were sacrificed on the 8th day to collect blood. The control group gave physiological saline. Blood component analysis was performed using an automatic blood cell counting device. In addition, the effect of WEM on peritoneal macrophages in BALB/c mice was studied. Intraperitoneal Injection of Sulfur B in Mice