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目的分析大肠癌患者含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)基因甲基化状态,寻找新的早期诊断大肠癌分子标志物。方法常规方法提取大肠癌标本DNA,采用甲基化特异性PCR技术分析TPEF基因甲基化状态。结果32例大肠癌手术切除标本,24例检测到异常TPEF基因甲基化(75%),而10例正常组织标本无一例检测到异常甲基化。结论大肠癌患者TPEF基因甲基化为一频繁发生事件,可望成为大肠癌早期诊断的一个标志物。
Objective To analyze the methylation status of transmembrane protein (TPEF) gene in patients with colorectal cancer, including epidermal growth factor and follistatin, and to find new molecular markers for early diagnosis of colorectal cancer. Methods The DNA of colorectal cancer samples was extracted by routine method. The methylation status of TPEF gene was analyzed by methylation - specific PCR. Results Thirty-two cases of colorectal cancer underwent surgical resection. Abnormal methylation of TPEF gene was detected in 24 cases (75%), while none of the 10 cases of normal tissue specimens detected abnormal methylation. Conclusion The methylation of TPEF gene in colorectal cancer patients is a frequent occurrence, which is expected to become a marker of early diagnosis of colorectal cancer.