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目的建立海南龙血竭的HPLC分析方法。方法采用对照品鉴定了17个化合物,并选取其中三种黄酮类成分进行含量测定。结果 4,4’-二羟基-2-甲氧基二氢查耳酮、4,4’-二羟基-2,6-二甲氧基二氢查耳酮、(2S)-7,3’-二羟基-4’-甲氧基黄烷浓度分别在10~50μg·ml-1(r=0.999 9)、24~120μg·ml-1(r=0.9995)、48~240μg·ml-1(r=0.999 8)内与各自峰面积积分值呈良好的线性关系;平均回收率分别为97.73%(RSD=0.02%)、97.46%(RSD=0.03%)、99.44%(RSD=1.12%)(n=5);成分含量分别为为2.2,1.0,2.2 mg/g。结论该方法精密度和重现性好,可用于海南龙血竭的质量控制。
Objective To establish a method for HPLC analysis of dried dragon’s blood in Hainan. Methods Seventeen compounds were identified by reference substance and three flavonoids were selected for determination. Results 4,4’-dihydroxy-2-methoxy dihydrochalcone, 4,4’-dihydroxy-2,6-dimethoxy dihydrochalcone, (2S) -7,3 ’ (R = 0.999 9), 24 ~ 120μg · ml-1 (r = 0.9995), 48 ~ 240μg · ml-1 (dihydroxy-4’-methoxyflavan) r = 0.999 8). The average recoveries were 97.73% (RSD = 0.02%), 97.46% (RSD = 0.03%) and 99.44% (RSD = 1.12% n = 5). The contents of the components were 2.2, 1.0 and 2.2 mg / g, respectively. Conclusion The method has good precision and reproducibility and can be used for the quality control of DRX in Hainan.