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采用染色体压片法和形态观察法,对大白菜hau胞质雄性不育材料6w-9605A的2个遗传世代(F1和BC6)的染色体数和花器官进行鉴定。结果表明:F1染色体数为28条;BC6染色体数为20条,与白菜的染色体数相同。6w-9605A的花药呈三角形,干瘪无花粉,败育彻底。利用芸薹属作物雄性不育基因线粒体保守序列设计3对引物,在6w-9605A中扩增出867bp的片段。基因预测显示,在线粒体基因atp6的下游产生1个新的开放阅读框为orf288,与目前报道的芸薹属作物细胞质雄性不育相关基因orf224、orf138等均不相同,这种新的基因结构特点极有可能就是大白菜hau胞质不育系6w-9605A产生不育的原因。
Chromosome number and flower organ of two genetic generations (F1 and BC6) of 6w-9605A, a Chinese cabbage hau cytoplasmic male sterile line, were identified by chromosome compression and morphological observation. The results showed that there were 28 F1 chromosomes and 20 BC6 chromosomes, the same as that of Chinese cabbage. 6w-9605A was triangular anthers, dry shriveled pollen, abortion thorough. Three pairs of primers were designed based on the mitochondrial conserved sequence of male sterile gene in Brassica crops and a 867 bp fragment was amplified in 6w-9605A. Gene prediction shows that there is a new open reading frame (ORF288) downstream of the atp6 mitochondrial gene, which is different from the orf224, orf138 gene related to cytoplasmic male sterility in Brassica crops reported at present. The new gene structural features Most likely is the Chinese cabbage hau cytoplasmic male sterile 6w-9605A causes of infertility.