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目的探讨N-乙酰氨基葡萄糖(N-Acetyl-D-glucosamine,GlcNAc)对应激小鼠模型腹腔巨噬细胞(peritoneal macrophage,PMφ)吞噬功能的调节作用。方法以足底电刺激法复制应激小鼠模型,将50只小鼠分为正常对照组、应激模型组、低剂量GlcNAc治疗组、中剂量GlcNAc治疗组和高剂量GlcNAc治疗组,每组10只。采用酵母吞噬法检测PMφ吞噬功能,MTT法检测PMφ能量代谢水平,使用原子力显微镜观察PMφ吞噬酵母的情况。结果与正常对照组相比,应激模型组PMφ吞噬功能明显下降(P<0.01),能量代谢水平明显下降(P<0.01),而中剂量和高剂量GlcNAc能有效提高巨噬细胞吞噬功能和调节细胞活性。结论GlcNAc可促进应激小鼠PMφ的吞噬而改善免疫功能。
Objective To investigate the regulatory effect of N-acetyl-D-glucosamine (GlcNAc) on the phagocytosis of peritoneal macrophage (PMφ) in a mouse model of stress. Methods Fifty mice were randomly divided into normal control group, stress model group, low dose GlcNAc treatment group, middle dose GlcNAc treatment group and high dose GlcNAc treatment group. Each group 10 only The phagocytosis of PMφ was detected by yeast phagocytosis, the energy metabolism of PMφ was detected by MTT assay, and the phagocytic yeast PMφ was observed by atomic force microscopy. Results Compared with the normal control group, the phagocytic function of PMφ was significantly decreased (P <0.01) and the energy metabolism was significantly decreased in the stress model group (P <0.01), while the middle dose and high dose of GlcNAc could effectively increase the phagocytic function of macrophages and Regulates cell activity. Conclusion GlcNAc can promote phagocytosis of PMφ in stress mice and improve immune function.