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以切花菊(Dendranthema morifolium)品种‘神马’为试材,外源喷施0.1 mmol·L-1的亚精胺(Spd)与多胺合成抑制剂D-精氨酸(D-Arg),转入昼10 h/夜14 h的短日条件下进行开花诱导,测定不同花芽分化时期顶芽内源多胺[腐胺(Put)、亚精胺(Spd)、精胺(Spm)]和几种激素[生长素(IAA)、玉米素核苷(ZR)、异戊烯基腺苷(iPA)、赤霉素(GA)]含量的动态变化,分析多胺对激素和花芽分化的作用关系。结果表明,外源多胺和多胺合成抑制剂能够显著影响顶芽内源多胺(Put、Spd、Spm)和激素(IAA、ZR、iPA、GA)的含量,顶芽内高水平多胺有利于菊花花芽分化的启动和保持;外源多胺及多胺合成抑制剂可能通过影响内源多胺含量从而影响内源激素或者直接影响内源激素和内源多胺,进而调控花芽分化:内源Put与IAA关系密切,高水平的内源Put不利于IAA的积累;ZR和iPA含量与内源多胺总量的变化趋势一致;外源多胺及多胺合成抑制剂对GA的影响主要在花序分化期和小花分化期,且高水平的内源Spd和Put不利于GA的积累。
In this experiment, Dendranthema morifolium cultivar ’Shenma’ was used as test material, and 0.1 mmol·L-1 spermidine (Spd) and polyamine synthesis inhibitor D-arginine (D-Arg) The flowering induction was carried out on the short day of 10 h / night and 14 h, and the endogenous polyamines (put, spermidine and spermine (Spm) The dynamic changes of the contents of several hormones [IAA, ZR, iPA, GA] and the effect of polyamines on hormone and floral bud differentiation relationship. The results showed that exogenous polyamines and polyamine synthesis inhibitors could significantly affect the content of endogenous polyamines (Put, Spd, Spm) and hormones (IAA, ZR, iPA, GA) Is conducive to the initiation and maintenance of flower bud differentiation; exogenous polyamines and polyamine synthesis inhibitors may affect the endogenous hormone through affecting the content of endogenous polyamines or directly affect the endogenous hormones and endogenous polyamines, thereby regulating flower bud differentiation: Endogenous Put was closely related to IAA, high level of endogenous Put was not conducive to the accumulation of IAA; ZR and iPA content trends with the trend of the total amount of endogenous polyamines; exogenous polyamines and polyamine synthesis inhibitors on GA Mainly in the inflorescence differentiation and floret differentiation, and high levels of endogenous Spd and Put is not conducive to the accumulation of GA.