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本研究以紫花苜蓿为寄主植物,采用五室培养系统按照根系在土壤中分布的体积比例定量模拟根系损伤,初步探讨紫花苜蓿和菌根真菌共生体的协同修复作用及其生态效应。结果表明,在不同的试验设计中,接种菌根有效地缓解了根系损伤带来的不利影响,双侧接菌在植株生物量、菌根侵染效果以及收集到的矿质元素浓度等均高于单侧接菌和对照,土壤有效磷含量在伤根前后仍维持在10 mg/kg。接菌改善了紫花苜蓿根际微环境,在一定程度上减缓根系损伤对植物的负面影响,有利于采煤沉陷区地表植被的修复。
In this study, the alfalfa was the host plant, and the five-compartment culture system was used to quantitatively simulate root damage according to the volume proportion of the root system distributed in the soil. The synergistic repair effect and its ecological effect of alfalfa and mycorrhizal fungi symbiosis were discussed. The results showed that in different experimental designs, inoculation mycorrhizal effectively alleviate the adverse effects of root damage. Bilateral bacterial inoculum in the plant biomass, mycorrhizal infection and the concentration of mineral elements collected were higher than Unilateral bacterial inoculation and control, soil available P content remained at 10 mg / kg before and after injury. Inoculation improved the rhizosphere microenvironment of alfalfa, and to a certain extent slowed down the negative impact of root damage on plants, which was beneficial to the restoration of surface vegetation in the mining subsidence area.