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本文利用测定G_1期和中S期细胞内放射性变化的方法(RC)测出小鼠KHT肉瘤的细胞周期时相的时间及其变异系数(CV)。腹腔注射~3H-UdR后,8小时再注射~(125)I-UdR,按2小时间隔取肿瘤制成单个细胞悬液,DNA特异性染料色霉素A_3染色,根据细胞DNA含量用FACS荧光激活细胞分类器分离出纯的G_1期和中S期细胞,分别测定细胞中~(125)I和~3H的放射性,用多室数学模型根据每个细胞内~(125)I和~3H的放射性变化,计算出TG_1为6.7小时,Ts为9.0小时,TG2M为3小时,生长指数为1。
In this paper, the time of cell cycle and the coefficient of variation (CV) of mouse KHT sarcoma were determined by measuring the intracellular radioactivity changes in G 1 and middle S phases (RC). After intraperitoneal injection of ~3H-UdR, ~125 I-UdR was injected at 8 hours. The tumors were taken at 2 hour intervals to make a single cell suspension. The DNA specific dye, chromomycin A_3, was stained and FACS fluorescence was used according to the cell DNA content. Activate the cell classifier to isolate pure G1 and middle S phase cells, and measure the radioactivity of ~(125)I and ~3H in the cells, using a multi-chamber mathematical model based on the ~(125)I and ~3H in each cell. Changes in radioactivity were calculated to be 6.7 hours for TG_1, 9.0 hours for Ts, 3 hours for TG2M, and a growth index of 1.