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在实验室条件下研究了不同浓度氯氰菊酯胁迫下,龙须菜的生长状况及藻体生理生化指标变化情况.结果表明,龙须菜对氯氰菊酯敏感性较低,氯氰菊酯浓度低于100μg.L-1时对龙须菜生长的抑制作用不明显.在氯氰菊酯胁迫的24h内,龙须菜体内的叶绿素a、藻红蛋白、可溶性蛋白、可溶性糖含量以及超氧化物歧化酶(SOD)活性均呈现出低浓度(<5或<10μg.L-1)促进、高浓度(>10或>50μg.L-1)抑制的现象.在本研究设置的氯氰菊酯浓度下(1～100μg.L-1),膜脂过氧化产物丙二醛(MDA)含量均增加,呈现典型的剂量-反应关系,由氧自由基所引起的膜脂过氧化是氯氰菊酯对龙须菜产生毒害作用的重要原因.低浓度暴露早期SOD的激活以及蛋白质和糖类的促进作用对于抵抗氯氰菊酯的过氧化胁迫具有重要作用,但在高浓度氯氰菊酯胁迫下,SOD的失活致使氯氰菊酯对龙须菜产生明显毒性作用.
Under the laboratory conditions, the growth of Gracilaria lemaneiformis under different concentrations of cypermethrin and the physiological and biochemical changes of algal biomass were studied.The results showed that Gracilaria lemaneiformis was less sensitive to cypermethrin and cypermethrin concentration was lower than 100μg.L-1 When the cypermethrin stress within 24h, the content of chlorophyll a, phycoerythrin, soluble protein, soluble sugar content and superoxide dismutase (SOD) activity of Gracilaria were shown (<10 or> 50μg.L-1) promoted by low concentration (<5 or <10μg.L-1) .In the cypermethrin concentration range set in this study (1-100μg.L-1) The content of malondialdehyde (MDA) in membrane lipid peroxidation products increased, showing a typical dose-response relationship, the membrane lipid peroxidation caused by oxygen free radicals is an important reason for the toxic effects of cypermethrin on asparagus. Early activation of SOD and promotion of proteins and carbohydrates play an important role in the resistance to cypermethrin. However, in the presence of high concentrations of cypermethrin, the inactivation of SOD caused cypermethrin to produce significant toxic effects on asparagus.