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目的:建立番石榴叶中桑黄素来苏糖苷和桑黄素阿拉伯糖苷的含量测定方法。方法:用Diamonsil C18色谱柱(250mm×4.6mm,5μm),以甲醇-0.1%磷酸溶液(35∶65)为流动相,流速为1.0mL·min-1,检测波长为355nm。结果:桑黄素来苏糖苷在0.06132～0.14308μg范围内呈良好的线性关系(r=0.9999);桑黄素阿拉伯糖苷在0.05844～0.13636μg范围内呈良好的线性关系(r=1),平均回收率分别为98.6%和100.7%,RSD分别为1.0%和1.7%。结论:本方法对番石榴叶质量标准的制定具有很好的参考价值。
Objective: To establish a method for the determination of licaverin and flavins arabinose in guava leaves. Methods: A Diamonsil C18 column (250mm×4.6mm, 5μm) was used. The mobile phase consisted of methanol-0.1% phosphoric acid solution (35∶65), the flow rate was 1.0mL·min-1, and the detection wavelength was 355nm. RESULTS: There was a good linear relationship (r=0.9999) in the range of 0.06132 to 0.14308 μg of licaverin and a good linear relationship (r=1) in the range of 0.05844 to 0.13636 μg of arbutin arabinoside. The rates were 98.6% and 100.7%, respectively, and the RSD was 1.0% and 1.7%, respectively. Conclusion: This method has a good reference value for the development of guava leaf quality standards.